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| Catalog Number | Size | Price |
|---|---|---|
| LS-G48121-100 | 100 µg | $480 |
Human CCNE1 / Cyclin E1 Protein (Recombinant His-GST, N-terminus) (Met1-Ala410) - LS-G48121
Human CCNE1 / Cyclin E1 Protein (Recombinant His-GST, N-terminus) (Met1-Ala410) - LS-G48121
Description:
CCNE1 / Cyclin E1 Protein LS-G48121 is a Recombinant Human CCNE1 / Cyclin E1 produced in Baculovirus Met1-Ala410 with His-GST, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
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Overview
Description:
CCNE1 / Cyclin E1 Protein LS-G48121 is a Recombinant Human CCNE1 / Cyclin E1 produced in Baculovirus Met1-Ala410 with His-GST, N-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
CCNE1 / Cyclin E1
Synonyms
CCNE1 | Cyclin E1 | CCNE | Cyclin Es | Cyclin Et | G1/S-specific cyclin-E1 | G1/s-specific cyclin e | Cyclin E
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His-GST, N-terminus
Region
Met1-Ala410
Predicted Molecular Weight
The recombinant human CCNE1/GST chimera consists of 647 amino acids and has a calculated molecular mass of 75 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
Expression System
Baculovirus
Source Species
Insect
Purification
> 82 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human HG3C-CCNE1 at 10 µg/ml (100 µl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 45.0-106.0 ng/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, 1mM GSH, pH 7.4, 5% trehalose, 5% mannitol 0.01% Tween80.
Reconstitution
It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CCNE1 / Cyclin E1
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Images
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular CCNE1 / Cyclin E1 Proteins
Source:
Baculovirus
Tag:
None
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
