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| Catalog Number | Size | Price |
|---|---|---|
| LS-G65036-20 | 20 µg (0.23 mg/ml) | $1,107 |
Human BRAF / B-Raf Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G65036
Human BRAF / B-Raf Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G65036
Description:
BRAF / B-Raf Protein LS-G65036 is a Recombinant Human BRAF / B-Raf produced in HEK 293 Cells with Myc-DDK (Flag) tag(s). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
BRAF / B-Raf Protein LS-G65036 is a Recombinant Human BRAF / B-Raf produced in HEK 293 Cells with Myc-DDK (Flag) tag(s). For Research Use Only
Specifications
Type
Recombinant Protein
Target
BRAF / B-Raf
Synonyms
BRAF | 94 kDa B-raf protein | B-raf | BRAF1 | B Raf | Proto-oncogene B-Raf | p94 | B-RAF1 | NS7 | ONCOGENE BRAF | RAFB1
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Myc-DDK (Flag)
Predicted Molecular Weight
84.3 kDa
AA Sequence
MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH(CloningSiteandTags:TRTRPLEQKLISEEDLAANDILDYKDDDDKV)
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 80% as determined by SDS-PAGE and Coomassie blue staining
Bio-Activity
Activity was measured in a homogeneous time-resolved fluorescent (HTRF) assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37°C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader.
Endotoxin
Not Tested
Presentation
Supplied in 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Storage
Store at -80°C for up to 12 months. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About BRAF / B-Raf
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Images
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Purified recombinant protein BRAF was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Purified recombinant protein BRAF was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Purified recombinant protein BRAF was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Purified recombinant protein BRAF was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
