Target
SARS-CoV-2 S1
Synonyms
2019-nCoV S1 Glycoprotein | Wuhan Coronavirus S1 Glycoprotein | SARS-Cov2 (COVID-19) S1 Glycoprotein | SARS-CoV-2 | SARS-Cov2 | COVID-19
Species
Coronavirus SARS-CoV-2
Conjugations
Unconjugated
Predicted Molecular Weight
76.5 kDa
Expression System
HEK 293 Cells
Purification
Greater than 90 % as determined by reducing SDS-PAGE.
Usage Summary
A DNA sequence encoding the 2019-nCoV spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 µg/mL (100 µL/well) can bind Recombinant 2019-nCoV S1 Protein (His Tag), the EC50 of Recombinant 2019-nCoV S1 Protein (His Tag) is 200-600 ng/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4
Storage
Long term: store at -20°C to -80°C for up to Lyophilized store for up to 12 months. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted store at less than -20°C for 3 months
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee