ELISA Kit Reagents and Accessories

ELISA Kit Component

5 Plates/$93; 10 Plates/$155

ELISA Kit Component

5 Plates/$90; 10 Plates/$145

Antigen Coating Buffer maximizes the absorption of antigens onto polystyrene plates and stabilizes their structure to enhance specific signal, save reagents, and extend the shelf life of coated plates.

100 ml/$66; 500 ml/$166; 1 l/$318

ELISA Blocking Buffer (Alkaline Phosphatase) is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. It is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.

100 ml/$64; 500 ml/$160; 1 l/$291

Alkaline Phosphatase Conjugate Stabilizer is a proprietary formulation to stabilize and dilute alkaline phosphatase conjugates for use in ELISA, Western blot, and other immunoassay applications. This formulation minimizes non-specific interactions and reduces background.

25 ml/$52; 100 ml/$88; 500 ml/$253; 1 l/$394

TMB Substrate (Super Sensitive) is a ready-to-use TMB substrate for microwell applications containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer. This substrate is our most sensitive TMB buffer. This sensitive, faster kinetic TMB substrate is approximately 40-fold more sensitive than our most popular substrate, TMB Substrate (ELISA).

100 ml/$68

TMB Substrate (ELISA) is a ready-to-use solution containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer that does not contain aprotic solvents. This substrate is ideal for use in all ELISAs where the target detection level is in the ng-pg/mL range and horseradish peroxidase (HRP) is the conjugated detection enzyme.

100 ml/$66

TMB Substrate (Low Sensitivity) is a ready-to-use solution containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer.

100 ml/$76

pNPP Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.

100 ml/$69

TMB Substrate (Immunoblotting) is a ready-to-use formulation containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer.

100 ml/$82

General Assay Diluent is a mammalian protein-based ELISA additive used to equalize the sample and standard matrices for a more accurate result. Large differences in matrix complexity environment between standard curve and sample wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. By helping to equalize matrix complexity, General Assay Diluent reduces non-specific interactions and interference.

500 ml/$228; 1 l/$295; 100 ml/$68

IgM-Reducing Assay Diluent is a specially formulated additive to address IgM-mediated conjugate bridging interference in problematic serum and plasma samples tested in antibody sandwich ELISA formats. IgM-Reducing Assay Diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues.

500 ml/$240; 1 l/$306; 100 ml/$68

Assay Diluent (Non-Mammalian) is formulated to address nonspecific binding issues with human, porcine, or bovine serum or plasma samples tested in antigen-down ELISA formats. This non-mammalian assay additive works to enhance the specific anti-analyte/antigen antibody signal without denaturing the plate-adsorbed antigen molecules.

100 ml/$68; 500 ml/$240; 1 l/$306

ELISA Assay Diluent (Antigen-Down) is formulated for use with serum and plasma samples tested in antigen-down ELISA formats. Proprietary additives have been included to reduce the level of nonspecific IgG adsorption to antigen-coated and blocked ELISA well surfaces, as well as enhancing the specific anti-analyte/antigen-antibody signal without denaturing the plate adsorbed antigen molecules.

500 ml/$245; 1 l/$372; 100 ml/$68

ELISA Blocking Buffer (General) contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide or protein antigen-down ELISA formats. This blocking buffer provides a long-term stabilizing environment for dried antigen or antibody coat proteins and minimizes nonspecific binding interactions during the assay process.

100 ml/$60; 500 ml/$145

Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. ELISA Blocking Buffer (Background Blocking) provides superior background-blocking performance without the use of conventional cross-reactive protein additives.

100 ml/$64; 500 ml/$160; 1 l/$291

BCIP/NBT Substrate is useful for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. BCIP/NBT Substrate reacts with AP yielding a dark blue reaction product.

100 ml/$82

Stop Solution is a ready-to-use liquid acidic stop solution suitable for use with our TMB substrates. This product is suitable for all endpoint ELISAs using a TMB substrate for color development.

100 ml/$46

Stop Solution (AP)is a dry powder for use with pNPP-based colorimetric substrates. This product is suitable for all endpoint ELISAs using an AP substrate for color development of an AP-conjugate.

100 ml/$51

Use Antibody Coating Buffer to save reagents, enhance specific signal, and extend the shelf life of coated plates. Antibody Coating Buffer maximizes the adsorption of monoclonal and polyclonal antibodies onto polystyrene plates for use in capture (sandwich) immunoassays by stabilizing the three-dimensional structure of the antibody for optimal immunoassay performance.

500 ml/$166; 1 l/$318; 100 ml/$66

General Serum Diluent is formulated for diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid and cell culture media, for testing in sandwich ELISA configurations. General Serum Diluent can also be used for dilution of many types of serum samples, including mammalian and chicken, tested in antigen-down ELISA formats.

500 ml/$228; 1 l/$295; 100 ml/$68

ELISA Wash Buffer, 10X effectively rinses microtiter plates between reagent addition steps of an ELISA to remove signal-altering debris yet preserve assay components.

500 ml/$135; 1 l/$257; 100 ml/$60

HRP Conjugate Stock Stabilizer is best for users who desire an all-purpose HRP conjugate diluent and HRP stabilizer. This formulation may serve as a base to add specialized components to address non-specific binding and interference. It also serves as an excellent concentrated buffer to store HRP conjugates.

500 ml/$586; 1 l/$1,102; 100 ml/$122

ELISA Plasma Sample Diluent (Antigen-Down) is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This novel mammalian-protein-buffered formulation provides an assay environment that minimizes non-specific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction.

100 ml/$68; 500 ml/$228; 1 l/$295

Antigen-Down HRP Conjugate Stabilizer is a unique formulation specifically designed for use with anti-IgG-HRP conjugates. This stabilizing diluent contains proprietary non-mammalian protein additives that will not interfere with anti-IgG-HRP conjugates used in for antigen-down immunoassays or sandwich ELISA formats.

100 ml/$138; 500 ml/$662; 1 l/$1,242