Target
PIK3CA / PI3K Alpha
Synonyms
PIK3CA | MCAP | p110-alpha | p110alpha | PI3K | PI3-kinase p110 subunit alpha | PI3-kinase subunit alpha | PI3Kalpha | MCM | MCMTC | PI3K-alpha | PtdIns-3-kinase subunit alpha | CLOVE
Reconstitution
Add distilled water/PBS to give a target peptide concentration of 1-5mg/mL, and sonicate. To any insoluble peptide add pure acetic acid to bring the concentration of acetic acid to 10% (v/v), and sonicate. If the peptide remains insoluble, add acetonitrile to 20% (v/v), and sonicate. If the peptide again remains insoluble, lyophilize the peptide and add neat DMF dropwise until the peptide dissolves. Dilute this solution slowly with water to give approximately 10% (v/v) DMF. If the peptide precipitates at any stage during this step, stop adding water and add a little more DMF until the peptide redissolves.
Purification
Greater than 85%
Recommended Storage
Store at -20°C for up to 1 year.
Usage
LS-E42035 is the immunizing peptide used to generate
LS-C356254. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Restrictions
For research use only. Intended for use by laboratory professionals.