Target
HSPA9 / Mortalin / GRP75
Synonyms
HSPA9 | Heat shock 70 kDa protein 9 | GRP-75 | Heat shock 70kD protein 9B | GRP75 | Mortalin-2 | Mortalin, perinuclear | Mot-2 | MOT2 | Peptide-binding protein 74 | MOT | MTHSP75 | p66-mortalin | PBP74 | CSA | HSPA9B | Mortalin
Reconstitution
Add distilled water/PBS to give a target peptide concentration of 1-5mg/mL, and sonicate. To any insoluble peptide add pure acetic acid to bring the concentration of acetic acid to 10% (v/v), and sonicate. If the peptide remains insoluble, add acetonitrile to 20% (v/v), and sonicate. If the peptide again remains insoluble, lyophilize the peptide and add neat DMF dropwise until the peptide dissolves. Dilute this solution slowly with water to give approximately 10% (v/v) DMF. If the peptide precipitates at any stage during this step, stop adding water and add a little more DMF until the peptide redissolves.
Purification
Greater than 85%
Recommended Storage
Store at -20°C for up to 1 year.
Usage
LS-E41844 is the immunizing peptide used to generate
LS-B16891. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Restrictions
For research use only. Intended for use by laboratory professionals.