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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5752-100 | 100 µg (0.5 mg/ml) | $485 |
PathPlus™ Monoclonal Mouse anti‑Human WT1 / Wilms Tumor 1 Antibody (clone 1E9, IHC, WB) LS‑B5752
PathPlus™ Monoclonal Mouse anti‑Human WT1 / Wilms Tumor 1 Antibody (clone 1E9, IHC, WB) LS‑B5752
Note: This antibody replaces LS-C133975
Antibody:
WT1 / Wilms Tumor 1 Mouse anti-Human Monoclonal (1E9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
WT1 / Wilms Tumor 1 Mouse anti-Human Monoclonal (1E9) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
WT1 is a transcription factor essential to normal development of the human urogenital system. It is expressed in the fetal kidney and mesothelium, as well as in certain malignancies. It can be used to identify ovarian carcinoma and mesothelioma; levels of WT1 expression can also indicate disease progression in acute myeloid lymphoma. Furthermore, it is a potential target for cancer vaccines. In normal tissue, WT1 is positive in fallopian tube epithelium, smooth muscle cells and endothelial cells (nuclear and weak cytoplasmic localization), and testis. In cancer, it is positive in Wilms' tumor, malignant mesotheliomas, and serous ovarian adenocarcinomas.
References: Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13824-9, PMID: 20631300; Cell. 1993 Aug 27;74(4):679-91, PMID: 8395349; Sexual Development. 2007;1(4):238-54, PMID: 18391535; Oncologist. 2012;17(2):250-9, PMID: 22291091; BMC Cancer. 2006 Apr 11;6:90, PMID: 16606472
Target
Human WT1 / Wilms Tumor 1
Synonyms
WT1 | GUD | EWS-WT1 | Amino-terminal domain of EWS | WAGR | WIT-2 | Wilms tumor protein | AWT1 | Wilms tumor 1 | WT33 | NPHS4 | WT-1
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
1E9
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
WT1 (NP_000369, 349 aa ~ 439 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human WT1
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About WT1 / Wilms Tumor 1
Validation
Anti-WT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-WT1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
PathPlus™ WT1 / Wilms Tumor 1 Antibody (clone 1E9) for IHC, WB/Western, ELISA LS-B5752 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Fluorophore-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Human
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
