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| Catalog Number | Size | Price |
|---|---|---|
| LS-B4011-50 | 50 µl (0.5 mg/ml) | $460 |
1 of 2
2 of 2
PathPlus™ Polyclonal Rabbit anti‑Human TYRP1 / gp75 Antibody (aa396‑445, IHC, WB) LS‑B4011
PathPlus™ Polyclonal Rabbit anti‑Human TYRP1 / gp75 Antibody (aa396‑445, IHC, WB) LS‑B4011
Note: This antibody replaces LS-C102088
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
TYRP1 is a melanocyte-specific protein involved in melanin synthesis. It functions to stabilize tyrosinase protein and modulate its catalytic activity. It also plays a role in the maintenance of melanosome structure and influences melanocyte proliferation and cell death. Mutations in this gene are associated with oculocutaneous albinism type 3 (OCA3). Expression of this protein in metastatic melanoma correlates with Breslow thickness and poor prognosis, and it may be an effective target of inhibition therapy. In immunohistochemistry of normal tissue, it has membranous positivity on melanocytes.
References: Pigment Cell Res. 2001 Dec;14(6):437-44, PMID: 11775055; Br J Cancer. 2011 Nov 22; 105(11): 1726–1732, PMID: 22045183
Target
Human TYRP1 / gp75
Synonyms
TYRP1 | B-PROTEIN | CAS2 | CATB | Glycoprotein 75 | Hstyrrp | gp75 | OCA3 | SHEP11 | TRP-1 | Tyrosinase-related protein 1 | TYRP | TYRRP | Catalase B | DHICA oxidase | Melanoma antigen gp75
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa396-445 of human TYRP1 (P17643, NP_000541). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Goat, Panda, Dog, Cat, Bat, Rabbit, Horse, Guinea pig, Chicken, Platypus (100%); Elephant, Bovine, Pig, Opossum, Zebra finch, Lizard (92%); Xenopus (85%); Pufferfish, Zebrafish (83%).
Epitope
aa396-445
Specificity
Human TYRP1
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B4011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4011 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TYRP1 / gp75
Validation
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
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LSBio Ratings
PathPlus™ TYRP1 / gp75 Antibody (aa396-445) for IHC, WB/Western LS-B4011 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
In vitro modeling of hyperpigmentation associated to neurofibromatosis type 1 using melanocytes derived from human embryonic stem cells. Jennifer Allouche, Nathalia Bellon, Manoubia Saidani, Laure Stanchina-Chatrousse, Yolande Masson, Anand Patwardhan, Floriane Gilles-Marsens, Cédric Delevoye, Sophie Domingues, Xavier Nissan, Cécile Martinat, Gilles Lemaitre, Marc Peschanski, Christine Baldeschi. Proceedings of the National Academy of Sciences of the United States of America. 2015 July;112:9034-9.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Frozen, Immunofluorescence, Flow Cytometry
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Flow Cytometry
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
Date Requested: 12/26/2024
