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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16192-0.05 | 0.05 ml | $460 |
PathPlus™ Monoclonal Mouse anti‑Human NANOG Antibody (Concentrated, IHC) LS‑B16192
PathPlus™ Monoclonal Mouse anti‑Human NANOG Antibody (Concentrated, IHC) LS‑B16192
Note: This antibody replaces LS-C757367
Antibody:
NANOG Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
NANOG Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
Nanog is a homeobox transcription factor involved in maintaining the pluripotency of embryonic stem cells by blocking cell differentiation. It also plays a role in cancer cell proliferation, epithelial-mesenchymal transition, apoptosis, and metastasis. It is expressed in stem cells, as well as in a variety of carcinomas, including those of the breast, ovary, stomach, bladder, and malignant melanomas.
References: Cell Res 2007 17:42; Cancer Biol Therapy 2016 17:1.
Target
Human NANOG
Synonyms
NANOG | Nanog homeobox | NANOGP8 | HNanog | Homeobox protein NANOG
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Recombinant human Nanog.
Applications
- IHC - Paraffin (1:100 - 1:200)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NANOG
Validation
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ NANOG Antibody (Concentrated) for IHC, ELISA LS-B16192 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human, Monkey
Applications:
IHC, Immunofluorescence, Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/20/2024
Date Requested: 11/20/2024
