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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2829-50 | 50 µg (1 mg/ml) | $440 |
PathPlus™ Monoclonal Mouse anti‑Human MUC16 / CA125 Antibody (clone X306, IHC, WB) LS‑B2829
PathPlus™ Monoclonal Mouse anti‑Human MUC16 / CA125 Antibody (clone X306, IHC, WB) LS‑B2829
Note: This antibody replaces LS-C58420
Antibody:
MUC16 / CA125 Mouse anti-Human Monoclonal (X306) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
MUC16 / CA125 Mouse anti-Human Monoclonal (X306) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
CA-125 (MUC16) is a mucin glycoprotein expressed by epithelial cells of endometrium, endocervix, pleura, pericardium, and fallopian tube peritoneum. Antibodies to this target are useful for identifying non-mucinous epithelial ovarian carcinomas, serous and endometrioid adenocarcinomas, Krukenberg tumors, and uterine leiomyomas. Elevated levels of serum CA-125 are correlated with the presence or recurrence of ovarian cancer. In immunohistochemistry, MUC16 is positive in the apical brush border of the fallopian tube epithelium, serous ovarian adenocarcinomas and mesotheliomas. It is negative in melanoma, lung and other adenocarcinomas.
References: Clin Onc 12:437, 2015; International Journal of Cancer. 2002. 98 (5): 737–40. doi:10.1002/ijc.10250, PMID: 11920644; Expert Review of Molecular Diagnostics. 2010. 10 (8): 1069–83, PMID: 21080822.
Target
Human MUC16 / CA125
Synonyms
MUC16 | CA125 ovarian cancer antigen | CA125 | CA-125 | MUC-16 | Mucin-16
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
X306
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
CA125 antigen purified from human ovarian carcinoma
Specificity
CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Recognizes the epitope specificity group A, similar to the OC125 antibody. May be used as a capture antibody in ELISA assays for CA125. May be used to detect CA125 in ELISA assays. Not suitable for conjugation to HRP.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: Validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 10 ug/ml.
Presentation
TBS, 0.09% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MUC16 / CA125
Validation
Anti-MUC16 / CA-125 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-MUC16 / CA-125 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
PathPlus™ MUC16 / CA125 Antibody (clone X306) for IHC, WB/Western, ELISA LS-B2829 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
