Human Melanoma Antigen NKI/C3

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

NKI/C3

Unconjugated

Ascites

Concentrated

This antibody recognizes a heterogeneous 25-110 kD glycoprotein that is located mainly in the inner side of membranes of cytoplasmic vesicles in melanoma cells. The antigen has a 25 kD unglycosylated precursor in formalin fixed and paraffin-embedded tissue sections. Cross-reactivity: The antibody reacts with some mucus producing tumors, carcinoids, carcinomas of the thyroid, mast cells, histiocytes in tumor regions and with cells with secretory functions such as salivary glands, bronchial glands, sweat glands, pancreas and prostate.

• IHC
• IHC - Paraffin (1:100)

• IHC - Frozen
• Western blot

The antibody should be used on formalin fixed paraffin embedded tissue sections, it is not advisable to use the antibody on frozen sections. Immunohistochemistry: Typical working dilution: 1:10-1:20. 60 minutes primary antibody incubation at 25 C. Standard ABC technique. Positive Control: Tonsil. Staining Pattern Cytoplasmic and membrane. Western Blot: Not recommended.

50 mM PBS, pH 7.4, 15 mM Sodium Azide, 1% BSA

Store at 2-8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.