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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16252-100 | 100 µg (200 µg/ml) | $525 |
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PathPlus™ Recombinant Monoclonal Mouse anti‑Human KRT19 / CK19 / Cytokeratin 19 Antibody (clone rKRT19/799, IHC, IF, WB) LS‑B16252
PathPlus™ Recombinant Monoclonal Mouse anti‑Human KRT19 / CK19 / Cytokeratin 19 Antibody (clone rKRT19/799, IHC, IF, WB) LS‑B16252
Note: This antibody replaces LS-C751242
Antibody:
KRT19 / CK19 / Cytokeratin 19 Mouse anti-Human Recombinant Monoclonal (rKRT19/799) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
KRT19 / CK19 / Cytokeratin 19 Mouse anti-Human Recombinant Monoclonal (rKRT19/799) Antibody
Application:
IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
KRT19 is a type 1 keratin, an intermediate filament that provides structural support and has cell cycle regulatory functions in epithelial cells. KRT19 is a common epithelial marker with cytoplasmic positivity in immunohistochemistry. It is used to identify neoplastic epithelial cells in a number of cancers, including breast carcinomas, thyroid papillary carcinomas, cholangiocarcinomas, endometrial cancers, urothelial and collecting duct renal cancers, Paget’s disease, pancreatic cancers, and epithelial odontogenic tumors. Additionally, it is a putative cancer stem cell marker in hepatocellular carcinoma. In normal tissue, KRT19 is positive in epithelia of the breast, GI tract, kidney, bile ducts, pancreatic ducts, salivary and sweat glands, prostate epithelium, placental trophoblasts, uterus, and tonsillar squamous epithelium.
References: Clin Cancer Res. 2015 Jul 1;21(13):3081-91, PMID: 25820415; Int J Mol Sci. 2018 May; 19(5): 1423, PMID: 29747452; Cancers (Basel). 2019 Jan; 11(1): 99, PMID: 30650643
Target
Human KRT19 / CK19 / Cytokeratin 19
Synonyms
KRT19 | CK19 | Cytokeratin 19 | Cytokeratin-19 | Keratin 19 | Keratin-19 | CK-19 | K19 | K1CS | Keratin, type I, 40-kd
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Non-Reactivity
Rat, Ferret
Clonality
IgG1
Recombinant Monoclonal
Clone
rKRT19/799
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified.
Also available Azide-free, Carrier-free.
Immunogen
Recombinant full-length human KRT19 protein. Antigen Molecular Weight: 40 kDa
Specificity
This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40 kDa . Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
Applications
- IHC - Paraffin (1:50)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blotting (1-2 ug/ml) - Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 min at RT - Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
About KRT19 / CK19 / Cytokeratin 19
Validation
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ KRT19 / CK19 / Cytokeratin 19 Antibody (clone rKRT19/799) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B16252 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/15/2024
Date Requested: 11/15/2024
