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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2489-50 | 50 µg (0.5 mg/ml) | $485 |
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PathPlus™ Polyclonal Goat anti‑Human Kinesin Heavy Chain / KIF5B Antibody (aa951‑963, IHC, WB) LS‑B2489
PathPlus™ Polyclonal Goat anti‑Human Kinesin Heavy Chain / KIF5B Antibody (aa951‑963, IHC, WB) LS‑B2489
Note: This antibody replaces LS-C34646, LS-C71382, LS-C71350
Antibody:
Kinesin Heavy Chain / KIF5B Goat anti-Human Polyclonal (aa951-963) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Kinesin Heavy Chain / KIF5B Goat anti-Human Polyclonal (aa951-963) Antibody
Application:
IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Bovine, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
KIF5B is a kinesin protein involved in organelle, macromolecule and RNA transport as well as intracellular movement. KIF5B function is co-opted in some cancers; in triple negative breast cancer (TNBC), it has high expression and drives epithelial mesenchymal plasticity, promoting tumor cell stem-ness, migration and TGF-Beta-mediated invasion. In lung cancers, a KIF5B-RET fusion has been detected at a rate of around 1.4%, and when present has high rates of expression in cancer versus absence in normal tissue. This fusion functions as an oncogene that regulates cell growth and differentiation through the PI3K-Akt and RAS-Raf-MAPK pathways. In immunohistochemistry of normal tissue, KIF5B has cytoplasmic positivity in all tissues throughout the body.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; EBioMedicine. Volume 45, July 2019, Pages 92-107, DOI: 10.1016/j.ebiom.2019.06.009; Onco Targets Ther. 2019; 12: 4533–4542, PMID: 31289444
Target
Human Kinesin Heavy Chain / KIF5B
Synonyms
KIF5B | Kinesin 1 (110-120kD) | KINH | KNS | Kinesin family member 5B | Kinesin heavy chain | KNS1 | UKHC | Kinesin-1 heavy chain | Ubiquitous kinesin heavy chain
Host
Goat
Reactivity
Human, Monkey, Bovine, Dog, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-QPVAVRGGGGKQV, from the C-Terminus of protein sequence according to NP_004512.1.
Epitope
aa951-963
Specificity
Human KIF5B.
Applications
- IHC - Paraffin (2.5 µg/ml)
- Western blot (0.5 - 1.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2489 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2489 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About Kinesin Heavy Chain / KIF5B
Validation
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ Kinesin Heavy Chain / KIF5B Antibody (aa951-963) for IHC, WB/Western LS-B2489 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Kinesin-1 (uKHC/KIF5B) is required for bidirectional motility of ER exit sites and efficient ER-to-Golgi transport. Gupta V, Palmer KJ, Spence P, Hudson A, Stephens DJ. Traffic (Copenhagen, Denmark). 2008 9:1850-66.
KIF5C, a kinesin motor involved in apical trafficking of MDCK cells. Astanina K, Jacob R. Cellular and molecular life sciences : CMLS. 2010 67:1331-42.
Customer Reviews (0)
Featured Products
Species:
Bovine, Chicken, Mammal, Sea urchin, Squid
Applications:
IHC, ICC, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Species:
Pig, Human, Mouse, Rat, Porcine
Applications:
IHC, IHC - Paraffin, ICC
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
