Human Isocitrate Dehydrogenase R132H Mutant

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein A/G purified

Concentrated. Also available Ready-to-Use.

IDH1 Peptide

Mutation R132H

• IHC - Paraffin (1:50)
• ELISA (1:100 - 1:200)

Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual Use: 1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes. 2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.) 3. Primary Antibody: Dilute antibody at 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature. 5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature. 6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. 7. Dehydrate and apply coverslip.

Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide

Store at 2°C to 8°C. Do not freeze.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.