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Catalog Number Size Price
LS-B16892-50 50 µl (1 mg/ml) $460 
HSPA9 / Mortalin / GRP75 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA9 / Mortalin / GRP75 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA9 / Mortalin / GRP75 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA9 / Mortalin / GRP75 Antibody - Immunofluorescent analysis of GRP75 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
HSPA9 / Mortalin / GRP75 Antibody - Western blot analysis of GRP75 expression in MCF7 (A); NIH3T3 (B); COS7 (C); mouse brain (D) whole cell lysates.
HSPA9 / Mortalin / GRP75 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA9 / Mortalin / GRP75 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA9 / Mortalin / GRP75 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
HSPA9 / Mortalin / GRP75 Antibody - Immunofluorescent analysis of GRP75 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
HSPA9 / Mortalin / GRP75 Antibody - Western blot analysis of GRP75 expression in MCF7 (A); NIH3T3 (B); COS7 (C); mouse brain (D) whole cell lysates.
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PathPlus™ Polyclonal Rabbit anti‑Human HSPA9 / Mortalin / GRP75 Antibody (IHC, IF, WB) LS‑B16892

PathPlus™ Polyclonal Rabbit anti‑Human HSPA9 / Mortalin / GRP75 Antibody (IHC, IF, WB) LS‑B16892

Note: This antibody replaces LS-C482240
Antibody:
HSPA9 / Mortalin / GRP75 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16892-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
HSPA9 / Mortalin / GRP75 Rabbit anti-Human Polyclonal Antibody
Application:
IHC-P, ICC, IF, WB, IP
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
HSPA9 (Mortalin, GRP75) is a heat shock protein involved in cell proliferation, stress response and mitochondria maintenance. HSPA9 is of interest in neurodegenerative diseases including Alzheimer’s and Parkinson’s disease; it may have protective chaperone functions in the brain that help degrade and maintain lowered levels of aggregated or improperly folded proteins. In immunohistochemistry, HSPA9 has granular cytoplasmic positivity in all tissues. References: Front Endocrinol (Lausanne). 2018; 9: 196, PMID: 29755410; J Alzheimers Dis. 2014;38(1):165-70. doi: 10.3233/JAD-130428, PMID: 23948933; J Alzheimers Dis. 2014;38(1):165-70, PMID: 23948933
Target
Human HSPA9 / Mortalin / GRP75
Synonyms
HSPA9 | Heat shock 70 kDa protein 9 | GRP-75 | Heat shock 70kD protein 9B | GRP75 | Mortalin-2 | Mortalin, perinuclear | Mot-2 | MOT2 | Peptide-binding protein 74 | MOT | MTHSP75 | p66-mortalin | PBP74 | CSA | HSPA9B | Mortalin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Immunogen
Recombinant full length human GRP75.
Specificity
Recognizes endogenous levels of GRP75 protein.
Applications
  • IHC - Paraffin (1:100)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:20 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HSPA9 / Mortalin / GRP75
P38646 NM_004134 NP_004125.3

Validation

HSPA9 / Mortalin / GRP75 Antibody - Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA9 / Mortalin / GRP75 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

HSPA9 / Mortalin / GRP75 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ HSPA9 / Mortalin / GRP75 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B16892 has an LSBio Rating of
Laboratory Validation Score (5)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/24/2024