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| Catalog Number | Size | Price |
|---|---|---|
| LS-B127-50 | 50 µg (1 mg/ml) | $515 |
![GLUP / PACRG Antibody - Anti-PACRG Antibody - Western Blot. Western blot of Affinity Purified anti-PACRG antibody shows detection of a band ~40 kD corresponding to human PACRG (arrowhead lane 1). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (lane 2). Approximately 35 ug of a mouse embryonic fibroblast (MEF) whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/pathplus-glup-pacrg-antibody-ls-b127/62847_29254.jpg)
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PathPlus™ Polyclonal Rabbit anti‑Human GLUP / PACRG Antibody (IHC, WB) LS‑B127
PathPlus™ Polyclonal Rabbit anti‑Human GLUP / PACRG Antibody (IHC, WB) LS‑B127
Note: This antibody replaces LS-C19127
Antibody:
GLUP / PACRG Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Chicken
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
GLUP / PACRG Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Chicken
Format:
Unconjugated, Unmodified
Specifications
Description
GLUP (PACRG) is a protein that is conserved across metazoans. In vertebrates, this gene is linked in a head-to-head arrangement with the adjacent parkin gene, which is associated with autosomal recessive juvenile Parkinson's disease. These genes are co-regulated in various tissues and they share a bi-directional promoter. Both genes are associated with susceptibility to leprosy. The parkin co-regulated gene protein forms a large molecular complex with chaperones, including heat shock proteins 70 and 90, and chaperonin components. This protein is also a component of Lewy bodies in Parkinson's disease patients, and it suppresses unfolded Pael receptor-induced neuronal cell death. Multiple transcript variants encoding different isoforms have been found for this gene.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human GLUP / PACRG
Synonyms
PACRG | GLUP | HAK005771 | PARK2 co-regulated | PARK2CRG | RP3-495O10.2 | PARK2 coregulated gene protein
Host
Rabbit
Reactivity
Human, Mouse, Chicken
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Chicken, Zebrafish (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human PACRG protein.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, mouse and chicken based on 100% homology for the immunogen sequence. Cross reactivity with PACRG protein from zebrafish and rat may occur as this sequence only varies by one amino acid residue (94% homology). Expect reactivity with both splice variants of this protein. Cross reactivity with PACRG homologues from other sources has not been determined.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Western blot (1:500 - 1:3000)
- ELISA (1:2000 - 1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B127 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B127 was determined to be 2.5 ug/ml.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GLUP / PACRG
Validation
Anti-PACRG antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PACRG antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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LSBio Ratings
PathPlus™ GLUP / PACRG Antibody for IHC, WB/Western, ELISA LS-B127 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
PARK2 and PACRG are commonly downregulated in clear-cell renal cell carcinoma and are associated with aggressive disease and poor clinical outcome. Toma MI, Wuttig D, Kaiser S, Herr A, Weber T, Zastrow S, Koch R, Meinhardt M, Baretton GB, Wirth MP, Fuessel S. Genes, chromosomes & cancer. 2013 Mar;52:265-73.
Customer Reviews (0)
Featured Products
Source:
E. coli
Tag:
His-GST
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/28/2024
Date Requested: 11/28/2024
