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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16545-0.05 | 0.05 ml | $460 |
PathPlus™ Monoclonal Mouse anti‑Human FSH Antibody (Concentrated, IHC) LS‑B16545
PathPlus™ Monoclonal Mouse anti‑Human FSH Antibody (Concentrated, IHC) LS‑B16545
Note: This antibody replaces LS-C759819
Antibody:
FSH Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
FSH Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
Follicle-stimulating hormone (FSH) is a glycoprotein hormone found in humans and other animals. The beta subunit (symbol FSHB) is unique to follicle stimulating hormone, while the alpha subunit (FSHA) is identical across chorionic gonadotropin, thyroid-stimulating hormone and luteinizing hormone. FSH is synthesized and secreted by gonadotrophs of the anterior pituitary gland, and regulates the development, growth, pubertal maturation, and reproductive processes of the body. FSH is implicated in proliferation and metastases in endometrial, prostate and ovarian cancers. In immunohistochemistry of normal tissue, FSH has positive cytoplasmic staining in pituitary tissue and in the placenta.
References: Oncol Lett. 2013 Oct;6(4):1128-1132, PMID: 24137476; J Clin Endocrinol Metab. 2004 Nov;89(11):5508-16, PMID: 15531506; Anticancer Res. 2009 May;29(5):1575-8, PMID: 19443368; Br J Cancer. 2000 Dec;83(12):1730-4, PMID: 11104573
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Human FSH beta.
Applications
- IHC - Paraffin (1:50)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual Use: 1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes. 2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.) 3. Primary Antibody: Dilute antibody at 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature. 5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature. 6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. 7. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pituitary: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ FSH Antibody (Concentrated) for IHC, ELISA LS-B16545 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
