FOXO1 is a transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. FOXO1 also modulates redox balance, bone mass and osteoblast numbers, and mediates the endocrine function of the skeleton in regulating glucose metabolism. It binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. It is an important regulator of cell death that acts downstream of CDK1, PKB/AKT1 and SKT4/MST1. It is required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner, and it promotes neural cell death. In immunohistochemistry, FOXO1 has variable levels of cytoplasmic and nuclear positivity in most tissues throughout the body. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804;