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| Catalog Number | Size | Price |
|---|---|---|
| LS-B7426-50 | 50 µg | $460 |
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PathPlus™ Polyclonal Rabbit anti‑Human FGF2 / Basic FGF Antibody (IHC, WB) LS‑B7426
PathPlus™ Polyclonal Rabbit anti‑Human FGF2 / Basic FGF Antibody (IHC, WB) LS‑B7426
Note: This antibody replaces LS-C104590, LS-C42360
Antibody:
FGF2 / Basic FGF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
FGF2 / Basic FGF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Specifications
Description
FGF2 (basic fibroblast growth factor) is an FGF family protein involved in angiogenesis, hematopoiesis and nervous system differentiation and function. FGF2 promotes smooth muscle cell growth, tissue repair and wound healing. FGF2 is potentially a useful marker for malignancy in oral squamous cell carcinoma, as expression is correlated with malignant transformation. In immunohistochemistry of normal tissue, FGF2 has nuclear and secreted positivity throughout the central nervous system, and is found in muscle, soft tissue, immune tissues, and at lower levels with primarily nuclear localization in other tissues.
References: Wiley Interdiscip Rev Dev Biol. 2015 May; 4(3): 215–266, PMID: 25772309; Endocrine Reviews. 1997. 18(1):26–45, DOI:10.1210/edrv.18.1.0292; PLoS One. 2015 Oct 14;10(10):e0138801, PMID: 26465941;
Target
Human FGF2 / Basic FGF
Synonyms
FGF2 | Basic fibroblast growth factor | FGFB | Fibroblast growth factor 2 | FGF-2 | Prostatropin | BFGF | HBGF-2
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated.
Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human FGF-basic.
Specificity
Human FGF-basic
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.1 - 0.2 µg/ml)
- ELISA (0.5 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B7426 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/ml), a concentration of 0.25-0.40 ug/ml of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (LS-C104313) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Reconstitute in sterile water to 0.1-1 mg/ml.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About FGF2 / Basic FGF
Validation
Anti-FGF2 / BFGF antibody IHC of human breast, myoepithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-FGF2 / BFGF antibody IHC of human breast, myoepithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
PathPlus™ FGF2 / Basic FGF Antibody for IHC, WB/Western, ELISA LS-B7426 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Bovine, Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Species:
Bovine, Human, Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot
Species:
Bovine, Human, Pig
Applications:
IHC, Western blot, Radioimmunoassay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/12/2024
Date Requested: 11/12/2024
