Features: - Mouse IgM primary antibody detection - Peroxidase enzyme detection system - Avidin/biotin complex (ABC) technology - Good sensitivity/low background - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Kit Contents: - 3 ml Normal Goat Serum - 1 ml Biotinylated Goat Anti-Mouse IgM - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated Peroxidase) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® ABC-HRP Staining Kit (Peroxidase, Mouse IgM) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document), which can be used to detect any molecule that is biotinylated. This kit consists of the Blocking Serum, a species specific Biotinylated Secondary Antibody, and the HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1009). Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting