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Catalog Number Size Price
LS-J1033-10 10 ml $211 
Product
Product - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.
Product - In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.
Product - FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.
Product
Product - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.
Product - In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.
Product - FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.
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VECTASHIELD Antifade Mounting Medium with DAPI - LS-J1033

VECTASHIELD Antifade Mounting Medium with DAPI - LS-J1033


Description:
A ready-to-use aqueous non-hardening mounting medium for long term storage without sealing. Inhibits photobleaching of fluorescent dyes and proteins, and contains DAPI counterstain.
Price
Catalog Number
$211
LS-J1033-10

Available for USA Shipment Only
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
A ready-to-use aqueous non-hardening mounting medium for long term storage without sealing. Inhibits photobleaching of fluorescent dyes and proteins, and contains DAPI counterstain.

Specifications

Features: - Ready-to-use aqueous solution - Contains DAPI counterstain (blue fluorescence staining of nuclei) - Non-hardening formulation - Inhibits photobleaching of fluorescent dyes and fluorescent proteins - No warming necessary - No sealing necessary for long term storage - Contains no counterstain - Ideal refractive index (1.45) - 10 ml volume - Six month expiration date - CE Mark Applications: - Immunofluorescence - In situ hybridization - Cellular Imaging Details: VECTASHIELD Antifade Mounting Medium with DAPI does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 °C, protected from light. This solution contains DAPI (4 , 6-diamidino-2-phenylindole), which produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm. The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD® Mounting Medium without DAPI (LS-F1032). VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. See the VECTASHIELD® Mounting Media Compatibility document to determine if VECTASHIELD® will be compatible in your system. Antifade Comparison: Other manufacturers measure the antifade properties of their mounts using labeled microspheres or arrayed spots. Vector® Labs prefers to measure antifade properties of VECTASHIELD® mounts using frozen tissue sections immunohistochemically stained with fluorescently labeled secondary antibodies. Antifade capability is measured using a 40x objective with real time imaging over 30 seconds of continuous exposure to the excitation illumination. Individual intensity measurements are recorded from 6 separate labeled regions and the average is calculated. The intensity after 30 second exposure is expressed as a percentage of the intensity at zero time. The values for PG are taken from the manufacturer s published results.


Restrictions
For research use only. Intended for use by laboratory professionals.
Available for shipment within the USA only


LSBio is a proud distributor of Vector Laboratories products

Reviews (0)

Images

Product

In situ hybridization

Product - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.
Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.

In situ hybridization

Product - In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.
In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.

In situ hybridization

Product - FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.
FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.

Product

In situ hybridization

Product - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.
Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.

In situ hybridization

Product - In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.
In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.

In situ hybridization

Product - FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.
FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.

Product

In situ hybridization

Product - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.
Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.

In situ hybridization

Product - In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.
In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.

In situ hybridization

Product - FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.
FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.

Product

In situ hybridization

Product - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.
Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD® Mounting Medium with DAPI (blue) - Photo courtesy of Cytocell Ltd.

In situ hybridization

Product - In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.
In situ hybridization using FastTag® FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red™ Substrate. Mounted in VECTASHIELD® Mounting Media with DAPI.

In situ hybridization

Product - FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.
FastTag® Biotin-labeled pUC1.77 detected with Texas Red®Avidin DCS, Biotinylated Anti-Avidin and Texas Red®Avidin DCS, and counterstained with VECTASHIELD® with DAPI.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com