Features: - Rat IgG primary antibody detection - Alkaline phosphatase enzyme detection system - Conjugated micropolymer technology - High sensitivity, low background - One-step detection - Enhanced accessibility to nuclear and membrane antigens - Ready-to-use in a convenient dropper bottle - Shorter assay time - Simplified multiple labeling - 15 ml and 50 ml sizes - Sufficient for staining approximately 75-150 (15 ml) or 250-500 (50 ml) sections Kit Contents: - 2.5% Normal Horse Serum for blocking (ready-to-use) - ImmPRESS®-AP (alkaline phosphatase) Polymer Anti-Rat IgG Reagent (ready-to-use) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - ELISA Details: The ImmPRESS® polymerized reporter enzyme staining system uses novel conjugation and micropolymer chemistries to create a highly sensitive, ready-to-use, one-step detection system. This unique micropolymer of highly active enzyme (peroxidase or alkaline phosphatase) is attached to our affinity purified secondary antibodies, producing reagents with outstanding sensitivity and low background. The ImmPRESS®-AP Alkaline Phosphatase Polymer Reagent micropolymer technology limits steric interference and provides enhanced accessibility to the target avoiding the disadvantages of other polymer systems that use large dextrans or other macromolecules as backbones. The result is crisp, strong staining of antibody targets, especially nuclear and membrane antigens, and greater sensitivity than conventional alkaline phosphatase antibody conjugates and other alkaline phosphatase polymer systems for immunohistochemistry and other applications. The staining procedure is simple. Following a blocking step with the diluted normal horse serum, sections are incubated with primary antibody. After a brief wash, the appropriate ImmPRESS® Reagent is added to the sections and incubated for 30 minutes. Sections are again rinsed and the slides are developed with the peroxidase substrate of choice. This kit provides the Serum Blocking, Secondary Antibody, and Enzyme Conjugate reagents for the following standard Immunohistochemistry protocol. See the list below to select the other necessary reagents. Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain - Mounting