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Home > Immunohistochemistry Reagents > LS-J1089
 
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Catalog Number Size Price
LS-J1089-1 1 Each $213 
Product
Product - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Product - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Product - Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Product - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Product - Tonsil (double label): CD3 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red).
Product - Colon (double label): M2A Antigen (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Product - Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red); Cytokeratin 8/18 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Product - Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP kit, Vector Blue™ substrate (blue).
Product - Pancreas (double label): Beta cells (blue, Vector® Blue), Alpha cells (brown, Vector® DAB).
Product - Uterus (double label): Epithelium (blue, Vector® Blue), muscle (red, Vector® Red).
Product - Epithelium: Proliferating cells (brown, DAB), endothelial cells (blue, Vector Blue™ substrate), epithelial cells (red, Vector Red™ Substrate).
Product - Tonsil: Cytokeratin (AE1/AE3 (m), ImmPRESS-AP Reagent, Vector Blue™ AP Substrate (blue).
Product - Melanoma was stained with anti-vimentin followed by ImmPRESS™-AP Anti-Rabbit IgG Reagent and Vector Blue™ Substrate. Note the excellent contrast of the substrate with the brown pigments.
Product - Breast Cancer (double Label): Epithelium (red, Vector® NovaRED), endothelium (blue, Vector® Blue).
Product - Breast Cancer (double label): Endothelium (red, Vector® NovaRED), epithelium (blue, Vector® Blue).
Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
Product - Colon (double label): M2A antigen (m), VECTASTAIN® ABC-AP Kit (Universal), Vector Blue™ (blue). CD20 (m), ImmPRESS™ Reagent (HRP, Universal), Vector® VIP (purple).
Product - Colon (double label): CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red).
Product - Tumor (double label): CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ AP substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red).
Product
Product - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Product - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Product - Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Product - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Product - Tonsil (double label): CD3 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red).
Product - Colon (double label): M2A Antigen (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Product - Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red); Cytokeratin 8/18 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Product - Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP kit, Vector Blue™ substrate (blue).
Product - Pancreas (double label): Beta cells (blue, Vector® Blue), Alpha cells (brown, Vector® DAB).
Product - Uterus (double label): Epithelium (blue, Vector® Blue), muscle (red, Vector® Red).
Product - Epithelium: Proliferating cells (brown, DAB), endothelial cells (blue, Vector Blue™ substrate), epithelial cells (red, Vector Red™ Substrate).
Product - Tonsil: Cytokeratin (AE1/AE3 (m), ImmPRESS-AP Reagent, Vector Blue™ AP Substrate (blue).
Product - Melanoma was stained with anti-vimentin followed by ImmPRESS™-AP Anti-Rabbit IgG Reagent and Vector Blue™ Substrate. Note the excellent contrast of the substrate with the brown pigments.
Product - Breast Cancer (double Label): Epithelium (red, Vector® NovaRED), endothelium (blue, Vector® Blue).
Product - Breast Cancer (double label): Endothelium (red, Vector® NovaRED), epithelium (blue, Vector® Blue).
Product - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN® Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Elite® ABC Kit, DAB substrate (brown).
Product - Prostate (double label): Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ (blue) substrate. CD34, VECTASTAIN® ABC-AP Kit, Vector Red™ (red) substrate.
Product - Tumor (double label): p53 (m), VECTASTAIN® ABC-AP Kit, Vector Blue™ substrate (blue); Multi-Cytokeratin (m), VECTASTAIN® Elite® ABC Kit, AEC substrate (red).
Product - Colon (double label): M2A antigen (m), VECTASTAIN® ABC-AP Kit (Universal), Vector Blue™ (blue). CD20 (m), ImmPRESS™ Reagent (HRP, Universal), Vector® VIP (purple).
Product - Colon (double label): CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), ImmPRESS™ Universal Reagent, Vector® NovaRED® substrate (red).
Product - Tumor (double label): CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ AP substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red).
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Vector® Blue Alkaline Phosphatase (Blue AP) Substrate Kit - LS-J1089

Vector® Blue Alkaline Phosphatase (Blue AP) Substrate Kit - LS-J1089

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Description:
This substrate produces a blue chromogen/blue fluorescent precipitant in the presence of Alkaline Phosphatase (AP) enzyme. Suitable for brightfield and darkfield visualization in IHC, ICC, and ISH, as well as various blotting applications. Sufficient to produce 200 ml of working solution.
Datasheet
Price
Catalog Number
$213
LS-J1089-1

Available for USA Shipment Only
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Reviews Images Related Products Request SDS/MSDS

Overview

Description:
This substrate produces a blue chromogen/blue fluorescent precipitant in the presence of Alkaline Phosphatase (AP) enzyme. Suitable for brightfield and darkfield visualization in IHC, ICC, and ISH, as well as various blotting applications. Sufficient to produce 200 ml of working solution.
Datasheet

Specifications

Features: - Blue chromogen /blue fluorescent precipitant - Fluorescent (Excitation: broad, peak at ~500 nm / Emission: broad, peak at ~680 nm) - Precipitated by Alkaline Phosphatase (AP) - Suitable for non-aqueous (permanent) mounting - Sufficient reagents to produce 200 ml of working solution - For Brightfield, Darkfield, Electron, Spectral Imaging - Suitable for IHC, ICC, IF, ISH, Blotting Applications, and Elispot - No wait times for mixing and dissolving powders or tablets - Suitable for use in single or multiple labeling assays - Heat Stable - Contrast with Tissue Pigments - One year expiry date Applications: - Immunohistochemistry - Immunocytochemistry - Immunofluorescence - In situ hybridization - Blotting Applications - Elispot Details: The Vector Blue™ AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications. With the aid of imaging systems and software, the spectral profile of this substrate can be distinguished from other enzyme substrates in applications where antigens are co-localized. Vector Blue™ is also fluorescent. Sections stained with the Vector Blue™ substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount) must be used because the reaction product is soluble in xylene. Vector Blue™ can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.


Restrictions
For research use only. Intended for use by laboratory professionals.
Available for shipment within the USA only

LSBio is a proud distributor of Vector Laboratories products

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Images

Product

Immunohistochemistry

Product - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Product - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Product - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Product

Immunohistochemistry

Product - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Product - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Product - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Product

Immunohistochemistry

Product - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Product - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Product - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Product

Immunohistochemistry

Product - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).
Mesothelioma (double label): M2A Antigen (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red); CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue).

Immunohistochemistry

Product - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).
Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD34 (m), VECTASTAIN® Universal Elite® ABC Kit, DAB substrate (brown).

Immunohistochemistry

Product - Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).
Tonsil (double label): Cyclin A (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ substrate (blue); CD20 (m), VECTASTAIN® Universal Elite® ABC Kit, Vector® NovaRED® substrate (red).

Immunohistochemistry

Product - Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).
Melanoma: Anti-Vimentin (rabbit mab), ImmPRESS-AP Anti-Rabbit IgG, Vector Blue™ Substrate (blue).

Related Products

Levamisole Solution - LS-J1030
Endogenous alkaline phosphatase (AP) and peroxidase (HRP) activities in frozen intestine revealed with Vector Red™ AP Substrate (red) and ImmPACT™ DAB HRP Substrate (brown) (left). Same substrates used on BLOXALL™ Solution-treated tissue (right). BLOXALL™ Block.
BLOXALL™ Endogenous Peroxidase and Alkaline Phosphatase Blocking Solution - LS-J1031
Tumor (double label): CD34 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Blue™ AP substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN® Universal ABC-AP Kit, Vector Red™ substrate (red).
Vector Red™ Alkaline Phosphatase (Red AP) Substrate Kit - LS-J1086
Prostate: Anti-PSA (mouse monoclonal), ImmPRESS™ AP Anti-Mouse Ig Reagent (LS-J1054), ImmPACT™ Vector Red™ Alkaline Phosphatase Substrate (LS-J1087), viewed under brightfield microscopy.
ImmPACT™ Vector Red™ Alkaline Phosphatase (AP) Substrate - LS-J1087
Colon Carcinoma: Pan-Cytokeratin (m), VECTASTAIN® ABC-AP Kit, Vector® Black (brown-black).
Vector® Black Alkaline Phosphatase (AP) Substrate Kit - LS-J1088

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

Orders:
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For technical assistance:
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