Features: - Complete double-label staining kits - Mouse (red) and rabbit (brown) IgG primary antibody detection - Peroxidase (HRP) and Alkaline Phosphatase (AP) enzyme detection system - ImmPRESS® amplified micropolymer technology (HRP and AP) - ImmPACT™ amplified DAB and Vector Red™ substrates - Convenient prediluted, ready-to-use reagents - Highest sensitivity, low background - Sufficient reagents to stain approximately 150 sections Kit Contents: - 15 ml BLOXALL™ Blocking Solution - 15 ml 2.5% Normal Serum - 15 ml ImmPRESS® Duet HRP/AP Polymer Reagent (mixture of ImmPRESS® HRP Anti-Rabbit IgG and ImmPRESS® AP Anti-Mouse IgG) - ImmPACT™ DAB EqV Substrate (HRP, brown) - ImmPACT™ Vector Red™ Substrate (AP, magenta) Applications: - Immunohistochemistry - Immunocytochemistry Details: Save time and streamline your double staining IHC workflow with the ImmPRESS® Duet Double Staining Polymer Kit. This kit enables fast, well-defined localization and visualization of two different target antigens on the same tissue section. Utilizing our unique ImmPRESS® micropolymer chemistry, we independently conjugate highly-active horseradish peroxidase (HRP) and alkaline phosphatase (AP) enzyme polymers to anti-mouse IgG and anti-rabbit IgG affinity-purified, highly cross-adsorbed secondary antibodies. These reagents are then blended at optimized dilutions to provide a stable pre-diluted, ready-to-use HRP/AP formula that will detect mouse and rabbit primary antibodies, and facilitate a time-saving, simplified, one-step double label detection protocol. For maximum sensitivity and staining contrast between target antigens, ImmPACT™ DAB EqV (HRP, brown) and ImmPACT™ Vector Red™ (AP, magenta) substrates are included to complete this easy to use staining kit.