Features: - Rat IgG primary antibody detection - Peroxidase enzyme detection system - Advanced Elite® Avidin/Biotin technology - Highest sensitivity, low background - Approximately 5 times more sensitive - Format: Concentrate - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 500-1000 sections Kit Contents: - 3 ml Normal Rabbit Serum - 1 ml Biotinylated Rabbit Anti-Rat IgG - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated Peroxidase) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Rat IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document) , which can be used to detect any molecule that is biotinylated. Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background. This kit consists of the Blocking Serum, a species specific Biotinylated Secondary Antibody, and the Elite® HRP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1018). Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting