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Catalog Number Size Price
LS-J1001-1 1 Each $520 
Product

VECTASTAIN® ABC-AP Staining Kit (Alkaline Phosphatase, Rabbit IgG) - LS-J1001

VECTASTAIN® ABC-AP Staining Kit (Alkaline Phosphatase, Rabbit IgG) - LS-J1001


Description:
This ABC kit is for the detection of rabbit IgG primary antibodies and is sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Contents include goat blocking serum, a biotinylated goat anti-rabbit IgG secondary antibody, Avidin DH, and biotinylated Alkaline Phosphatase.
Price
Catalog Number
$520
LS-J1001-1

Available for USA Shipment Only
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
This ABC kit is for the detection of rabbit IgG primary antibodies and is sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Contents include goat blocking serum, a biotinylated goat anti-rabbit IgG secondary antibody, Avidin DH, and biotinylated Alkaline Phosphatase.

Specifications

Features: - Rabbit IgG primary antibody detection - Alkaline phosphatase enzyme detection system - Avidin/biotin complex (ABC) technology - High sensitivity/low background - Preferred for tissues that have high endogenous peroxidase activity - Offers additional substrate choices - Used as a second label in multiple antigen labeling applications - Sufficient for staining approximately 1000-2000 sections or fifty 100 cm2 blots. Kit Contents: - 3 ml Normal Goat Serum - 1 ml Biotinylated Goat Anti-Rabbit IgG - 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated AP) Applications: - Immunohistochemistry - Immunocytochemistry - In situ hybridization - Blotting Applications - Elispot - ELISA Details: The VECTASTAIN® ABC-AP Staining Kit (Alkaline Phosphatase, Rabbit IgG) is part of the modular VECTASTAIN® ABC system (see the VECTASTAIN® ABC System document), which can be used to detect any molecule that is biotinylated. This kit consists of the Blocking Serum, a species-specific Biotinylated Secondary Antibody, and the AP Enzyme Conjugate reagents listed in the following standard Immunohistochemistry protocol. Alternatively, this kit is also available without the Blocking Serum or species-specific Biotinylated Secondary Antibody (LS-J1000). The sensitivity of the VECTASTAIN® ABC-AP system is comparable to that of the peroxidase VECTASTAIN® Elite® ABC system (HRP). The VECTASTAIN® ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices. Immunohistochemistry protocol: Fixation – Histology - Antigen Retrieval – Endogenous Block – Serum Block -- Primary Antibody - Secondary Antibody – Enzyme Conjugate – Substrate - Counter-stain – Mounting


Restrictions
For research use only. Intended for use by laboratory professionals.
Available for shipment within the USA only


LSBio is a proud distributor of Vector Laboratories products

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