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| Catalog Number | Size | Price |
|---|---|---|
| LS-F5014-1 | 1 Plate | $806 |
1 of 2
2 of 2
Bovine TNF Alpha (Sandwich ELISA) ELISA Kit - LS-F5014
Bovine TNF Alpha (Sandwich ELISA) ELISA Kit - LS-F5014
Description:
LS-F5014 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Bovine TNF Alpha in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of TNF Alpha as low as 7.8 picograms per milliliter.
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
LS-F5014 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Bovine TNF Alpha in samples of Cell Culture Supernatants, Cell Lysates, Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of TNF Alpha as low as 7.8 picograms per milliliter.
Specifications
Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Reactivity
Bovine
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Manual
Format
96-Well Strip Plate
Intended Sample Type
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
7.8 - 500 pg/ml
Sensitivity
7.8 pg/ml
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This ELISA Kit carries the LSBio 100% Guarantee
About TNF Alpha
LSBio Ratings
Bovine TNF Alpha (Sandwich ELISA) ELISA Kit - LS-F5014 has an LSBio Rating of
Publications (4.3)
Learn more about The LSBio Ratings Algorithm
Publications (4)
Effects of arginase inhibition via jugular infusion of N?-hydroxy-nor-l-arginine on metabolic and immune indices in lactating dairy cows. L Y Ding, Y F Wang, Y Z Shen, G Zhou, X Zhang, M Z Wang, J J Loor, J Zhang. Journal of dairy science. 2019 Apr;102:3310-3320.
Histamine promotes adhesion of neutrophils by inhibition of autophagy in dairy cows with subacute ruminal acidosis. Kexin Wang, Zhenai Sun, Yunfei Li, Mingchao Liu, Juan J Loor, Qianming Jiang, Guowen Liu, Zhe Wang, Yuxiang Song, Xinwei Li. Journal of dairy science. 2022 September;105:7600-7614.
Free fatty acids promote degranulation of azurophil granules in neutrophils by inducing production of NADPH oxidase-derived reactive oxygen species in cows with subclinical ketosis. Yuxiang Song, Shang Jiang, Congyi Li, Juan J Loor, Qianming Jiang, Yuchen Yang, Xiancheng Feng, Siyuan Liu, Jiyuan He, Kexin Wang, Yunfei Li, Cai Zhang, Xiliang Du, Zhe Wang, Xinwei Li, Guowen Liu. Journal of dairy science. 2022 March;105:2473-2486.
Cellular and mitochondrial adaptation mechanisms in the colon of lactating dairy cows during hyperthermia. Mehdi Eslamizad, Dirk Albrecht, Björn Kuhla, Franziska Koch. Journal of dairy science. 2023 December;
Customer Reviews (0)
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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview
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Reactivity:
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31.25-2000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/21/2024
Date Requested: 11/21/2024
