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Catalog Number Size Price
LS-K283-100 100 Tests $530 
IDH / Isocitrate Dehydrogenase Assay Kit

Isocitrate Dehydrogenase (IDH) Assay Kit (Colorimetric) - LS-K283

Isocitrate Dehydrogenase (IDH) Assay Kit (Colorimetric) - LS-K283

Available for shipment within the USA only
Description:
ISOCITRATE DEHYDROGENASE (IDH) is an enzyme which catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. This non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Price
Catalog Number
$530
LS-K283-100

Available for USA Shipment Only
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
ISOCITRATE DEHYDROGENASE (IDH) is an enzyme which catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. This non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Specifications

Name
Isocitrate Dehydrogenase (IDH) Assay Kit (Colorimetric)
Type
Detection/Quantition
Usage
For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism.
Target
IDH / Isocitrate Dehydrogenase
SampleType
Culture Media, Plasma, Serum, Tissue
Detection
Colorimetric (565 nm Absorbance)
Supplied Components
The following components are supplied with this product.
  • Assay Buffer
  • Diaphorase
  • NADP/MTT
  • Calibrator
  • Substrate
  • (See Datasheet for specific volumes supplied)
Applications
Spectrophotometry (visible)
Equipment
Microplate spectrophotometer (visible)
Conditions
Shipped Ambient, Store at -20°C, 6 months shelf life.
Documents
Restrictions
For research use only. Intended for use by laboratory professionals. Available for shipment within the USA only
Guarantee
This Assay Kit carries the LSBio 100% Guarantee

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Colorimetry

IDH / Isocitrate Dehydrogenase Assay Kit

Colorimetry

IDH / Isocitrate Dehydrogenase Assay Kit

Colorimetry

IDH / Isocitrate Dehydrogenase Assay Kit

Colorimetry

IDH / Isocitrate Dehydrogenase Assay Kit

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 1/14/2025