Target
Human ZNF112 / ZFP112
Synonyms
ZNF112 | ZFP112 | ZNF228 | Zinc finger protein 228 | Zfp-112
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-EREDDIENSHLKSYQ, from the internal region of the protein sequence according to NP_001076804.1NP_037512.3.
Specificity
Human ZFP112 / ZNF228. This antibody is expected to recognize both reported isoforms (NP_001076804.1; NP_037512.3)
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx. 48kDa band in lysates of cell line HepG2 after 2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 105kDa according to NP_037512.3. The 48kDa band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee