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Catalog Number	Size	Price
LS-C154839-100	100 µg (0.5 mg/ml)	$503

ZIC2 Antibody - ZIC2 antibody RWPE1 lysate (60 ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with (0.5 ug/ml) in the right panel and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel. Mock-transfected RWPE1 in lanes 1 and expressing GFP in lanes 2. Primary incubations were overnight at 4C. Detected by chemiluminescence.

Polyclonal Goat anti‑Human ZIC2 Antibody (aa520‑531, WB) LS‑C154839

Name: Anti-ZIC2 Antibody | Goat anti-Human Polyclonal Peptide-ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C154839-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

ZIC2 Goat anti-Human Polyclonal (aa520-531) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Guinea pig

Format:

Unconjugated, Unmodified

$503

LS-C154839-100

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For Research Use Only

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Overview

Antibody:

ZIC2 Goat anti-Human Polyclonal (aa520-531) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Monkey, Mouse, Rat, Bat, Guinea pig

Format:

Unconjugated, Unmodified

Specifications

Description

ZIC2 antibody LS-C154839 is an unconjugated goat polyclonal antibody to ZIC2 (aa520-531) from human. It is reactive with human, mouse, rat and other species. Validated for Peptide-ELISA and WB.

Target

Human ZIC2

Synonyms

ZIC2 | HPE5 | Zic family member 2 | Zinc finger protein ZIC 2

See All ZIC2 Antibodies

Host

Goat

Reactivity

Human, Monkey, Mouse, Rat, Bat, Guinea pig (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-HSGLSSNFNEWY, from the C-Terminus of protein sequence according to NP_009060.2.

Epitope

aa520-531

Specificity

Human ZIC2. This antibody is expected to NOT cross-react with other ZIC proteins.

Applications

Western blot (0.5 - 2 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:1000)

Usage

Peptide ELISA: antibody detection limit dilution 1:1000. Western blot: In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kD is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kD according to NP_009060.2 and 58.6kD for the ZIC2-Myc protein. Data provided by Nathan Bowen and Maryam Boseman, Clark Atlanta University, GA, USA. We have observed an approx 75kD band in Human Brain (Cerebellum and Cerebral Cortex) lysates Recommended concentration, 0.5-2 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About ZIC2

Uniprot: O95409 NCBI: NM_007129 NP_009060.2