Reactivity
Yeast
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Immunogen
Rub1 Antibody was prepared from rabbit serum after repeated immunizations with full-length recombinant yeast Rub1 protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:1000 - 1:5000)
Usage
This purified polyclonal antibody reacts with yeast Rub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. A 6 kD band corresponding to yeast Rub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee