Target
Human XBP1
Synonyms
XBP1 | X-box binding protein 1 | X-box-binding protein 1 | XBP2 | TREB5 | XBP-1
Reactivity
Human, Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human XBP1 (59-85 aa RKRQRLTHLSPEEKALRRKLKNRVAAQ), identical to the related mouse and rat sequences.
Specificity
Expressed in plasma cells in rheumatoid synovium (PubMed:11460154). Over-expressed in primary breast cancer and metastatic breast cancer cells (PubMed:25280941). Isoform 1 and isoform 2 are expressed at higher level in proliferating as compared to confluent quiescent endothelial cells (PubMed:19416856). .
Applications
- IHC
- IHC - Paraffin (0.5 - 1 µg/ml)
- Western blot (0.1 - 0.5 µg/ml)
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Usage
WB: The detection limit for XBP1 is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee