Target
Human VCL / Vinculin
Synonyms
VCL | CMD1W | CMH15 | Metavinculin | Vinculin | MVCL
Reactivity
Human, Mouse, Chicken
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 1065. The sequence is conserved in mouse and chicken.
Specificity
Chicken and human vinculin. Mouse vinculin (100% homologous) has not been tested, but is expected to react.
Applications
- Western blot (1:1000)
- Immunoprecipitation
Usage
Suitable for use in Western blotting applications following immunoprecipitation. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. Positive control: COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA were treated with vanadate for 24 hr.
Presentation
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee