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Catalog Number Size Price
LS-C744702-100 100 µg (1 mg/ml) $552 
Uricase Antibody - Western Blot of Goat anti-Uricase Peroxidase Conjugated antibody. Lane 1: purified Uricase under reducing conditions. Lane 2: purified Uricase under reducing conditions. Lane 3: purified Uricase under non-reducing conditions. Lane 4: purified Uricase under non-reducing conditions Load: ~1.0 µg (Lane 1 and 3) and 0.25 µg (Lane 2 and 4). Primary antibody: Uricase Peroxidase Conjugated Antibody at 1:5,000 overnight at 4°C. Secondary antibody: DyLight 649 goat secondary antibody at 1:10,000 for 90 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 58 kDa, 58 kDa for Uricase. Other band(s): Uricase splice variants and isoforms.

Polyclonal Goat anti‑Bacillus Uricase Antibody (Lyophilized, HRP, WB) LS‑C744702

Polyclonal Goat anti‑Bacillus Uricase Antibody (Lyophilized, HRP, WB) LS‑C744702

Antibody:
Uricase Goat anti-Bacillus Polyclonal (Lyophilized) (HRP) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bacillus
Format:
Horseradish Peroxidase, Lyophilized
Price
Catalog Number
$552
LS-C744702-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Uricase Goat anti-Bacillus Polyclonal (Lyophilized) (HRP) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bacillus
Format:
Horseradish Peroxidase, Lyophilized

Specifications

Description
Uricase antibody LS-C744702 is an HRP-conjugated goat polyclonal antibody to bacillus Uricase. Validated for ELISA, IP and WB.
Target
Bacillus Uricase
Host
Goat
Reactivity
Bacillus (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Anti-uricase Antibody was produced by repeated immunizations with bacillus uricase protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Uricase [Bacillus species]. Cross reactivity against Uricase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation (1:100)
  • ELISA (1:1000 - 1:2500)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/25/2024