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Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Yeast
(tested or 100% immunogen sequence identity)
Clonality
IgGPolyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast ULP-1 protein.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Reactivity against ULP-1 from other sources or ULP-2 has not been determined.
Applications
Immunofluorescence
Western blot (1:500 - 1:2000)
ELISA (1:4000 - 1:20000)
Usage
This purified polyclonal antibody reacts with yeast ULP-1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 72.4 kD in size corresponding to yeast ULP-1 by western blotting in the appropriate lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.