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Catalog Number Size Price
LS-C482458-50 50 µl (1 mg/ml) $286 
LS-C482458-100 100 µl (1 mg/ml) $321 
LS-C482458-200 200 µl (1 mg/ml) $386 
UBCH10 / UBE2C Antibody - Immunohistochemical analysis of UBE2C staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
UBCH10 / UBE2C Antibody - Immunofluorescent analysis of UBE2C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
UBCH10 / UBE2C Antibody - Western blot analysis of UBE2C expression in THP1 (A); SW620 (B); SKOV3 (C) whole cell lysates.
UBCH10 / UBE2C Antibody - Immunohistochemical analysis of UBE2C staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
UBCH10 / UBE2C Antibody - Immunofluorescent analysis of UBE2C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
UBCH10 / UBE2C Antibody - Western blot analysis of UBE2C expression in THP1 (A); SW620 (B); SKOV3 (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human UBCH10 / UBE2C Antibody (IHC, IF, WB) LS‑C482458

Polyclonal Rabbit anti‑Human UBCH10 / UBE2C Antibody (IHC, IF, WB) LS‑C482458

Antibody:
UBCH10 / UBE2C Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482458-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
UBCH10 / UBE2C Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
UBE2C antibody LS-C482458 is an unconjugated rabbit polyclonal antibody to UBE2C (UBCH10) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human UBCH10 / UBE2C
Synonyms
UBE2C | DJ447F3.2 | UBCH10 | Ubiquitin-protein ligase C | Ubiquitin carrier protein E2-C | Ubiquitin carrier protein C
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Recombinant full length Human UBE2C.
Specificity
Recognizes endogenous levels of UBE2C protein.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UBCH10 / UBE2C
O00762 NM_007019 NP_008950.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024