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Catalog Number Size Price
LS-C413500-100 100 µl (1 mg/ml) $267 
TUBB3 / Tubulin Beta 3 Antibody - Immunohistochemical analysis of Beta-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TUBB3 / Tubulin Beta 3 Antibody - Immunofluorescent analysis of Beta-tubulin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TUBB3 / Tubulin Beta 3 Antibody - Western blot analysis of Beta-tubulin expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.
TUBB3 / Tubulin Beta 3 Antibody - Immunohistochemical analysis of Beta-tubulin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TUBB3 / Tubulin Beta 3 Antibody - Immunofluorescent analysis of Beta-tubulin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
TUBB3 / Tubulin Beta 3 Antibody - Western blot analysis of Beta-tubulin expression in HeLa (A); mouse brain (B); rat brain (C) whole cell lysates.
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Monoclonal Mouse anti‑Human TUBB3 / Tubulin Beta 3 Antibody (IHC, IF, WB) LS‑C413500

Monoclonal Mouse anti‑Human TUBB3 / Tubulin Beta 3 Antibody (IHC, IF, WB) LS‑C413500

Antibody:
TUBB3 / Tubulin Beta 3 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$267
LS-C413500-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TUBB3 / Tubulin Beta 3 Mouse anti-Human Monoclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Tubulin Beta 3 antibody LS-C413500 is an unconjugated mouse monoclonal antibody to Tubulin Beta 3 (TUBB3) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human TUBB3 / Tubulin Beta 3
Synonyms
TUBB3 | CDCBM | Beta III Tubulin | Class III beta-tubulin | TUBB4 | Tubulin, beta 3 | Tubulin beta-III | Tubulin beta-3 chain | Tubulin beta-4 chain | Tubulin, beta 3 class III | CFEOM3A
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Beta-tubulin.
Specificity
Recognizes endogenous levels of Beta-tubulin protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:200)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:5000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TUBB3 / Tubulin Beta 3
Q13509 NM_006086 NP_006077.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/12/2024