Target
Human TUBB3 / Tubulin Beta 3
Synonyms
TUBB3 | CDCBM | Beta III Tubulin | Class III beta-tubulin | TUBB4 | Tubulin, beta 3 | Tubulin beta-III | Tubulin beta-3 chain | Tubulin beta-4 chain | Tubulin, beta 3 class III | CFEOM3A
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Sheep
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Synthetic peptide corresponding to amino acids 443-450, ESESQGPK, of human class lll beta-tubulin.
Specificity
Recognizes the beta III isoform of human tubulin which is specific for neurons. Species cross-reactivity: mouse, avian, porcine, rat, bovine, sheep and monkey. Does not identify beta tubulin found in glial cells.
Applications
- IHC
- IHC - Paraffin
- ICC
- Western blot (1:1000)
- Immunoprecipitation
- ELISA
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Usage
Suitable for use in Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Immunocytochemistry (Fixed): 1:100-1:500. Immunohistochemistry: 1:20-1:100. Paraffin embedded, formalin fixed tissue. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections. Western Blot: 1:1000. Positive control: Brain tissue or neurons of any type.
Presentation
PBS, 15 mM Sodium Azide, 1% BSA
Storage
Short term: 4°C; Long term: -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TUBB3 / Tubulin Beta 3