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Catalog Number Size Price
LS-C662792-10 10 µg $318 
LS-C662792-100 100 µg $470 
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Western blot analysis of TSPAN12 using anti-TSPAN12 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPAN12 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSPAN12 at approximately 43KD. The expected band size for TSPAN12 is at 35KD.
TSPAN12 Antibody - Western blot - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Immunohistochemistry - Anti-TSPAN12 Picoband antibody
TSPAN12 Antibody - Western blot analysis of TSPAN12 using anti-TSPAN12 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPAN12 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TSPAN12 at approximately 43KD. The expected band size for TSPAN12 is at 35KD.
TSPAN12 Antibody - Western blot - Anti-TSPAN12 Picoband antibody
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Polyclonal Rabbit anti‑Human TSPAN12 Antibody (IHC, WB) LS‑C662792

Polyclonal Rabbit anti‑Human TSPAN12 Antibody (IHC, WB) LS‑C662792

Antibody:
TSPAN12 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662792-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TSPAN12 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TSPAN12 antibody LS-C662792 is an unconjugated rabbit polyclonal antibody to human TSPAN12. Validated for ELISA, IHC and WB.
Target
Human TSPAN12
Synonyms
TSPAN12 | EVR5 | NET-2 | NET2 | Tetraspan NET-2 | Tetraspanin-12 | TM4SF12 | Tspan-12 | Tetraspanin 12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived human TSPAN12 recombinant protein (Position: G111-R224).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TSPAN12
O95859 NM_012338 NP_036470.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/26/2024