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Catalog Number Size Price
LS-C681623-50 50 µg $294 
LS-C681623-100 100 µg $360 
TSPAN12 Antibody - Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
TSPAN12 Antibody - Immunofluorescence staining of A549 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
TSPAN12 Antibody - Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
TSPAN12 Antibody - Immunofluorescence staining of A549 cells diluted at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Polyclonal Rabbit anti‑Human TSPAN12 Antibody (IHC, IF) LS‑C681623

Polyclonal Rabbit anti‑Human TSPAN12 Antibody (IHC, IF) LS‑C681623

Antibody:
TSPAN12 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C681623-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TSPAN12 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
TSPAN12 antibody LS-C681623 is an unconjugated rabbit polyclonal antibody to human TSPAN12. Validated for ELISA, IF and IHC.
Target
Human TSPAN12
Synonyms
TSPAN12 | EVR5 | NET-2 | NET2 | Tetraspan NET-2 | Tetraspanin-12 | TM4SF12 | Tspan-12 | Tetraspanin 12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, HRP, FITC.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Tetraspanin-12 protein (111-224AA)
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TSPAN12
O95859 NM_012338 NP_036470.1

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Requested From: United States
Date Requested: 12/26/2024