Target
Human TRIM6
Synonyms
TRIM6 | RING finger protein 89 | RNF89 | Tripartite motif containing 6 | Tripartite motif-containing 6
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence KQLKAVLCADHGEK, from the internal region of the protein sequence according to NP_001003818.1NP_477514.1.
Specificity
Human TRIM6 / RNF89. This antibody is expected to recognize reported isoforms 1 and 2 (NP_001003818.1; NP_477514.1). Reported variants represent identical protein: NP_001185573.1, NP_001185574.1
Applications
- Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Usage
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 70-75kD band in Human Kidney and Liver lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.4kD according to NP_001003818.1. The 70-75kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee