Target
Human TPSAB1 / Mast Cell Tryptase
Synonyms
TPSAB1 | Alpha tryptase | Mast cell alpha II tryptase | Tryptase alpha-1 | Tryptase beta I | Tryptase-III | TPSB1 | Tryptase alpha II | TPS1 | TPS2 | Tryptase beta-1 | Tryptase-1 | Tryptase-I | Mast cell beta I tryptase | Tryptase alpha/beta 1 | Tryptase alpha/beta-1 | Tryptase beta 1 | Tryptase I
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Alkaline Phosphatase
Purification
Ion exchange chromatography
Immunogen
Human mast cell tryptase isolated from human lung tissues.
Specificity
Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.
Applications
- IHC (0.5 - 1 µg/ml)
- Western blot
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Usage
Suitable for use in Western Blot, Immunohistochemistry. Immunohistochemistry: ~0.5-1 ug/ml (1:1000-1:2000). For optimal results, tissues or cytospin preparations should be fixed in Carnoy's Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultra structural analysis after fixation of tissue in a modified Karnovsky's reagent (Lab Invest. (1988). 58:682-691). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
0.05 M Tris, 0.1 M sodium chloride, 0.001 M magnesiuim chloride, pH 8.0, 15mg/ml BSA, 0.09% sodium azide
Storage
Do not freeze. Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing will result in a substantial loss of activity.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TPSAB1 / Mast Cell Tryptase