Human TP53 / p53

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

BP53-12

Unconjugated

Protein A purified

Unmodified

This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.

Reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.

• IHC
• IHC - Paraffin (2.5 µg/ml)
• Immunofluorescence (1:100 - 1:500)
• Western blot (1:500 - 1:2000)
• Immunoprecipitation (1:100)
• ELISA (1:2000 - 1:10000)
• Chromatin Immunoprecipitation (1 µg/µl)

Immunohistochemistry: LS-B431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B431 was determined to be 2.5 ug/ml.

0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04637 NCBI: NM_000546 NP_000537.3