Target
Human TNNI3 / Cardiac Troponin I
Synonyms
TNNI3 | CTnI | CMD1FF | CMD2A | RCM1 | Troponin I, cardiac | Troponin I, cardiac muscle | Troponin I type 3 (cardiac) | Cardiac troponin I | CMH7
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-KVTKNITEIAD, from the internal region of the protein sequence according to NP_000354.4.
Specificity
Human TNNI3 / CTnI
Applications
- Immunofluorescence (10 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 27+28kD bands observed in Human Heart lysates (calculated MW of 24.0kD according to NP_000354.4). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.2-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNNI3 / Cardiac Troponin I