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Antibodies > TNK2 / ACK1 > LS-C804441
 
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Catalog Number Size Price
LS-C804441-100 100 µl (1 mg/ml) $379 
LS-C804441-200 200 µl (1 mg/ml) $421 
TNK2 / ACK1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
TNK2 / ACK1 Antibody - Western blot analysis of Phospho-Ack1 (Tyr857/858) using HeLa whole cells lysates
TNK2 / ACK1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
TNK2 / ACK1 Antibody - Western blot analysis of Phospho-Ack1 (Tyr857/858) using HeLa whole cells lysates
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Polyclonal Rabbit anti‑Human TNK2 / ACK1 Antibody (phospho‑Tyr857,phospho‑Tyr858, IF, WB) LS‑C804441

Polyclonal Rabbit anti‑Human TNK2 / ACK1 Antibody (phospho‑Tyr857,phospho‑Tyr858, IF, WB) LS‑C804441

Submit Review LSBio Guarantee
Antibody:
TNK2 / ACK1 Rabbit anti-Human Polyclonal (pTyr857,pTyr858) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C804441-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TNK2 / ACK1 Rabbit anti-Human Polyclonal (pTyr857,pTyr858) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ACK1 antibody LS-C804441 is an unconjugated rabbit polyclonal antibody to human ACK1 (TNK2) (pTyr857,pTyr858). Validated for IF, Peptide-ELISA and WB.
Target
Human TNK2 / ACK1
Synonyms
TNK2 | ACK | Activated p21cdc42Hs kinase | Ack kinase | ACK-1 | ACK1 | Activated CDC42 kinase 1 | p21cdc42Hs
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Phospho-Ack1 (Tyr857/858).
Epitope
pTyr857,pTyr858
Specificity
Phospho-Ack1 (Tyr857/858) Antibody detects endogenous levels of Ack1 only when phosphorylated at Tyr857/858.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNK2 / ACK1
Q07912 NM_005781 NP_005772.3

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Species: Human
Applications: Western blot, ELISA
TNK2 / ACK1 Antibody - Dot blot of anti-Phospho-ACK1-pY518 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
TNK2 / ACK1 Antibody (phospho‑Tyr518) LS‑C97107
Species: Human
Applications: Dot Blot
TNK2 / ACK1 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
TNK2 / ACK1 Antibody (aa934‑964) LS‑C99164
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
TNK2 / ACK1 Antibody - Anti-TNK2 / ACK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 1.25 ug/ml.
IHC‑plus™ TNK2 / ACK1 Antibody (aa278‑289) LS‑B6360
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
TNK2 / ACK1 Antibody - Immunohistochemical analysis of ACK1 (pY284) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
TNK2 / ACK1 Antibody (phospho‑Tyr284) LS‑C358365
Species: Human, Mouse, Rat, Bovine, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
TNK2 / ACK1 Antibody - Western blot analysis of lysates from HepG2 cells treated with EGF, using phospho-ACK (Phospho-Tyr518) antibody.
TNK2 / ACK1 Antibody (phospho‑Tyr518) LS‑C291479
Species: Human, Rat
Applications: Western blot, ELISA, Peptide Enzyme-Linked Immunosorbent Assay
See all TNK2 / ACK1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 2/12/2025
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