Mouse TNFSF9 / CD137L

Rat

Mouse (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

203942

Unconjugated

Protein G purified

Unmodified

Sf 21-derived rm4-1BB Ligand extracellular domain.

Extracellular Domain

Recognizes mouse 4-1BB Ligand. In direct ELISA, does not cross-react with rhRELT.

• Western blot (1 - 2 µg/ml)
• Flow Cytometry
• ELISA

Western Blot, Flow Cytometry, Direct ELISA. Western Blot-This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect mouse 4-1 BB Ligand. The detection limit for rm4-1BB Ligand is approximately 1 ng/lane under non-reducing and reducing conditions. Flow Cytometry-Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA-This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse 4-1 BB Ligand. The detection limit for rm4-1BB Ligand is approximately 1.5 ng/well.

Lyophilized from PBS, 5% Trehalose

Reconstitute with 1 ml sterile PBS.

Lyophilized and resuspended forms: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P41273 NCBI: NM_003811 NP_003802.1