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Catalog Number	Size	Price
LS-C661869-100	100 µg	$470

TNFSF14 / LIGHT Antibody - Western blot analysis of LIGHT using anti-LIGHT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates,Lane 2: mouse cardiac muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIGHT antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LIGHT at approximately 26KD. The expected band size for LIGHT is at 26KD.

TNFSF14 / LIGHT Antibody - Western blot - Anti-LIGHT Picoband Antibody

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Polyclonal Rabbit anti‑Mouse TNFSF14 / LIGHT Antibody (WB) LS‑C661869

Name: Anti-TNFSF14 / LIGHT Antibody | Rabbit anti-Mouse Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C661869-100
Price: 470.0000 USD
Availability: InStock

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Antibody:

TNFSF14 / LIGHT Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$470

LS-C661869-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TNFSF14 / LIGHT Rabbit anti-Mouse Polyclonal Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

LIGHT antibody LS-C661869 is an unconjugated rabbit polyclonal antibody to mouse LIGHT (TNFSF14). Validated for ELISA and WB.

Target

Mouse TNFSF14 / LIGHT

Synonyms

See All TNFSF14 / LIGHT Antibodies

Host

Rabbit

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived mouse LIGHT recombinant protein (Position: L59-V239).

Applications

Western blot
ELISA

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TNFSF14 / LIGHT

Uniprot: O43557 NCBI: NM_003807 NP_003798.2