Human TLR3

Mouse

Human, Mouse, Canine (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Low Endotoxin

Human Flag-tagged TLR3 stably expressed by Ba/F3 cells

• IHC
• IHC - Paraffin
• IHC - Frozen
• Immunofluorescence
• Immunoprecipitation
• Flow Cytometry
• Functional Assay

F: dried sections, fixed with 4% paraformaldehyde and subsequently washed in PBS and MQ. Sections were quenched with 0.3%H2O2 in methanol and washed in PBS. Sections were permeabilized with 0.4% triton-X100 in PBS. Pretreated slides were blocked with 1% horse serum for 20' and incubated o/n with antibody (Ref.5). FC: cells were incubated with 1 ug antibody together with 10 ug human IgG for 30' at 4°C in PBS/0,5% BSA (Ref.3). FS: 7.5*104 MRC5 cells were pre-treated with 10-20ug/ml for 1-24h at 37°C. Monoclonal antibody TLR3.7 inhibits dsRNA-induces IFN-beta production (Ref.1). IF: Cytospins of monocyte-derived iDCs were fixed for 30' with 3% formaldehyde in PBS, permeabilized with PBS/1%BSA/0.5%saponin. After PBS wash slides were incubated for 1h at RT with 20ug/ml antibody in PBS/1%BSA (Ref.3). P: Formalin fixed, paraffin embedded sections were deparaffinized with xylene, followed by washes in 95% and 70% EtOH. Sections were washed with water and permeabilized with 0.4% triton-X100 in PBS. Pretreated slides were blocked with 1% horse serum for 20' and incubated o/n with antibody (Ref.5). W: Total cellular protein was loaded on 7.5% SDS-PAGE and blotted on PDVF. Blots were incubated with 2ug/ml antibody o/n at 4°C (Ref.6).

Store at 4°C for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15455 NCBI: NM_003265 NP_003256.1