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Catalog Number	Size	Price
LS-C482462-50	50 µl (1 mg/ml)	$286
LS-C482462-100	100 µl (1 mg/ml)	$321
LS-C482462-200	200 µl (1 mg/ml)	$386

TLE1 / TLE 1 Antibody - Immunohistochemical analysis of TLE1 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

TLE1 / TLE 1 Antibody - Immunofluorescent analysis of TLE1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

TLE1 / TLE 1 Antibody - Western blot analysis of TLE1 expression in MCF7 (A); HT29 (B); mouse liver (C); mouse brain (D) whole cell lysates.

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Polyclonal Rabbit anti‑Human TLE1 / TLE 1 Antibody (IHC, IF, WB) LS‑C482462

Name: Anti-TLE1 / TLE 1 Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C482462-50
Price: 286.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

TLE1 / TLE 1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$286

LS-C482462-50

$321

LS-C482462-100

$386

LS-C482462-200

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

TLE1 / TLE 1 Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, ICC, IF, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

TLE 1 antibody LS-C482462 is an unconjugated rabbit polyclonal antibody to TLE 1 (TLE1) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.

Target

Human TLE1 / TLE 1

Synonyms

TLE1 | E(Sp1) homolog | ESG | GRG1 | Enhancer of split groucho 1 | ESG1

See All TLE1 / TLE 1 Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

Recombinant full length Human TLE1.

Specificity

Recognizes endogenous levels of TLE1 protein.

Applications

IHC
IHC - Paraffin (1:50 - 1:200)
ICC (1:50 - 1:200)
Immunofluorescence (1:50 - 1:200)
Western blot (1:500 - 1:2000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About TLE1 / TLE 1

Uniprot: Q04724 NCBI: NM_005077 NP_005068.2