Target
Human TJP3 / ZO3
Synonyms
TJP3 | Tight junction protein 3 | ZO-3 | Zona occludens protein 3 | Zonula occludens protein 3 | Tight junction protein ZO-3 | ZO3
Reactivity
Human, Dog
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide derived from the mid region of the human ZO-3 protein.
Specificity
Recognizes human ZO-3 protein with a molecular weight of ~130kD. Cross-reactivity with other Zonula Occluden family members has not been observed. Reactivity is confirmed with human T84, HeLa, and canine MDCK cell lysates.
Applications
- Immunofluorescence (0.5 - 2 µg/ml)
- Western blot (1 - 3 µg/ml)
- Immunoprecipitation
- ELISA (0.1 - 1 µg/ml)
Usage
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 0.5-2 ug/ml. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml. Immunoprecipitation: 2-3 ug.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee